Figure 4.

Phosphorylation sites of FKBP52 by CK2. (A) GST (lanes 1 and 4), GST-FKBP52 (amino acids 6–148) (lanes 2 and 5), and GST-FKBP52 (amino acids 32–138) (lanes 3 and 6) were phosphorylated by CK2 in vitro without thrombin cleavage. CBB staining (lanes 1–3) and autoradiography (lanes 4–6) are shown. (B) The amino acid sequence of the corresponding region of rabbit FKBP52. The result in A indicates that the boxed regions should contain the CK2-phosphorylation site(s), whereas the underlined region should not. Nonconsensus serines are indicated by arrowheads, and Thr-143, which agrees well with the CK2-phosphorylatable motif, is in bold type and indicated by an arrow.