Figure 5.

Phosphorylation of the Thr-143-containing peptide by CK2. Recombinant FKBP52 (amino acids 6–458) (A) or FKBP52 (amino acids 6–148) (B) was phosphorylated by CK2 in the presence of Pept419 or Pept790. The phosphorylation mixtures were analyzed by CBB staining (Upper) or by autoradiography (Lower). (C) Phosphorylation of Pept419 by CK2. Pept419-KLH or Pept790-KLH was phosphorylated in vitro by CK2. CBB staining (lanes 1 and 2) and autoradiography (lanes 3 and 4) are shown. The position of the peptide-conjugates is indicated by an arrow.