Figure 1.

CTL and proliferative responses of naive GM-CSF +/+ and GM-CSF −/− spleen cells against BALB/c spleen cells. Spleen cells from GM-CSF +/+ (+/+) or GM-CSF −/− (−/−) mice were stimulated in vitro with mitomycin C-treated BALB/c spleen cells. (a) Cytotoxicity was assessed with ⁵¹Cr-labeled BALB/c RL♂1 (○) and C57BL EL4 (•) target cells. (b) Spleen cells from GM-CSF +/+ or GM-CSF −/− mice were stimulated in vitro with mitomycin C-treated autologous (▧) or BALB/c (▨) spleen cells. Proliferation was determined by incorporation of [methyl-³H]thymidine.