Figure 2.

Generation of CTL against OVA peptide. Spleen cells of GM-CSF +/+ (a) or GM-CSF −/− mice (b and c) immunized with OVA peptide were stimulated with autologous spleen cells pulsed with OVA peptide and treated with mitomycin C. Cytotoxicity was assessed with ⁵¹Cr-labeled C57BL EL4 target cells pulsed with OVA peptide (○) or with no peptide (•). (c) The hind footpads of GM-CSF −/− mice were injected with 5 ng rGM-CSF and OVA peptide, followed by 10 ng/day rGM-CSF injected i.p. for 5 days, resulting in the generation of OVA-specific CTL .