Figure 4.

CD4⁺ T cell proliferative responses to KLH, immobilized anti-CD3 mAb, and Con A following immunization with KLH in CFA. (a) Purified CD4⁺ T cells from draining lymphnodes of immunized GM-CSF −/− or GM-CSF +/+ mice were stimulated with spleen cells pulsed with KLH (▨) or without KLH (▧) as indicated. Spleen cells were pulsed with 100 μg/ml KLH for 1 h. (b) Purified CD4⁺ T cells from immunized GM-CSF −/− (•) or GM-CSF +/+ (○) mice were stimulated with immobilized anti-CD3 mAb or Con A in vitro. Proliferation was determined by incorporation of [methyl-³H]thymidine.