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. 2008 Jul 24;6:41. doi: 10.1186/1479-5876-6-41

Figure 2.

Figure 2

FACS of yeast-display scFv library enriched for case-specific scFv. A naïve yeast-display scFv library was enriched by magnetic and fluorescent cell sortings for scFv that bound to case-pool serum. The enriched yeast-display scFv were labeled with anti-c-myc mAb and 25 μg/ml (A,B,E,F) or 100 μg/ml (C,D,G,H) of biotinylated case (A,C,E,G) or control (B,D,F,H) serum pools, before (A-D) or after depletion by magnetic sorting (E-H). Binding signals were detected as shown with the secondary antibody 488-alexa anti-mouse Ig (488 anti-mIg) and PE-labeled streptavidin (SA-PE).