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In situ hybridization of midbrain-hindbrain junction markers on sagittal sections of Pax-2 −/−, Pax-5 −/−, and wild-type e14 mouse embryos. None of the marker expression patterns shows variation in the mutants (C57BL/6) as compared with wild type. 4v, fourth ventricle; CB, cerebellum; tc, midbrain tectum; tg, midbrain tegmentum; is, isthmus constriction. Neither Pax-2 in the Pax-2 mutant nor Pax-5 in the Pax-5 mutant are shown (gray squares).
