Figure 3.

Early loss of midbrain/hindbrain tissue in Pax-2/Pax-5 compound mutant mice. Early deletion of midbrain/hindbrain junction tissue shown by β-galactosidase staining in e8.5 mouse embryos (12 somites) carrying the Pax-5-lacZ allele. (A) The Pax-2 +/+ Pax-5 −/− embryo show normal expression of the Pax-5-lacZ allele in the midbrain/hindbrain junction. (B) The compound Pax-2/Pax-5 mutant is missing the tissue of the midbrain/hindbrain junction as shown by the lack of β-galactosidase staining. Arrowhead in A points to lacZ-expressing tissue. Arrowhead in B points to the presumptive midbrain/hindbrain junction region. The dotted lines follow the ventral side of the rostral neural tube. FB, forebrain; HB, hindbrain; MB, midbrain.