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. Author manuscript; available in PMC: 2009 Jan 8.
Published in final edited form as: Curr Biol. 2008 Jul 8;18(13):969–975. doi: 10.1016/j.cub.2008.05.047

Figure 3. Respiration signaling does not solely occur via Rim101 regulation.

Figure 3

A) Rim101-3HA protein levels were determined in WT (A15869) and pet100Δ (A15871) cells incubated in KAc for the indicated times. Arrow: full-length Rim101, bracket: cleaved Rim101, which regularly appears as a doublet in KAc medium. Pgk1 is shown as a loading control.

B) Cells from (A) were pelleted and the pH of the supernatant was measured.

C) IME1-LacZ activity was measured in WT (A15869), pet100Δ (A15871), or rim101Δ (A18590) cells in saturated YPD cultures (time 0) or after incubation in KAc for the indicated times.

D) The number of nuclei per cell of cultures shown in (C) was determined by DAPI staining after 24 hours.