Table 2.

Effects of hypoxia on gene expression in mammary glands of nursing rats

Gene	Gene symbol	Normoxia	Hypoxia	p-value
nerve growth factor	Ngf	27.48 ± 0.24	27.94 ± 0.20	p> 0.05
solute carrier family 2 (facilitated glucose transporter), member 4	Slc2a4	26.23 ± 0.15	26.37 ± 0.24	p> 0.05
leptin	Lep	27.50 ± 0.29	28.08 ± 0.16	p> 0.05
solute carrier family 16 (monocarboxylic acid transporter), member 8	Slc16a8	22.89 ± 0.30	22.86 ± 0.08	p> 0.05
thyroid hormone receptor alpha	Thra	21.53 ± 0.13	21.45 ± 0.10	p> 0.05
thyroid hormone receptor beta	Thrb	22.83 ± 0.34	22.64 ± 0.12	p> 0.05
solute carrier family 5 (sodium iodide symporter), member 5	Slc5a5	25.30 ± 0.10	24.44 ± 0.13	p< 0.001
hydroxysteroid 11-beta dehydrogenase 1	Hsd11b1	25.38 ± 0.34	25.13 ± 0.21	p> 0.05
hydroxysteroid 11-beta dehydrogenase 2	Hsd11b2	27.55 ± 0.19	27.47 ± 0.22	p> 0.05
adiponectin	Adipoq	22.82 ± 0.43	22.17 ± 0.28	p> 0.05
ribosomal protein L19	Rpl19	18.03 ± 0.29	17.93 ± 0.16	p> 0.05

The data reported indicate the PCR cycle at which a threshold was reached (C_t). The SDS software automatically set the threshold line in the exponential phase of the amplifi cation. A lower C_t value indicates an increase in mRNA expression due to hypoxia. Data are presented as mean ± SEM (n = 8 samples per treatment; each sample run in triplicate in PCR assay)