Table 3.

Effects of pair feeding on gene expression in mammary glands of nursing rats

Gene	Gene symbol	Normoxia food ad libitum	Normoxia pair fed	p-value
epidermal growth factor	Egf	21.56 ± 0.17	21.76 ± 0.06	p> 0.05
solute carrier family 2 (facilitated glucose transporter), member 4	Slc2a4	25.61 ± 0.14	25.29 ± 0.05	p< 0.04
leptin	Lep	27.09 ± 0.27	27.36 ± 0.40	p> 0.05
solute carrier family 16 (monocarboxylic acid transporter), member 8	Slc16a8	21.81 ± 0.12	21.96 ± 0.06	p> 0.05
thyroid hormone receptor alpha	Thra	22.32 ± 0.06	21.77 ± 0.08	p< 0.001
thyroid hormone receptor beta	Thrb	21.81 ± 0.07	21.18 ± 0.08	p< 0.001
solute carrier family 5 (sodium iodide symporter), member 5	Slc5a5	24.97 ± 0.13	25.11 ± 0.12	p> 0.05
hydroxysteroid 11-beta dehydrogenase 1	Hsd11b1	25.50 ± 0.06	24.82 ± 0.08	p< 0.001
hydroxysteroid 11-beta dehydrogenase 2	Hsd11b2	27.88 ± 0.08	27.51 ± 0.09	p< 0.02
parathyroid hormone-like peptide	Pthlh	23.67 ± 0.45	24.49 ± 0.28	p> 0.05
ribosomal protein L19	Rpl19	16.32 ± 0.32	16.09 ± 0.32	p> 0.05

The data reported indicate the PCR cycle at which a threshold was reached (C_t). The SDS software automatically set the threshold line in the exponential phase of the amplification. A lower C_t value indicates an increase in mRNA expression due to pair feeding. Data are presented as mean ± SFM (n = 8-9 samples per treatment; each sample run intriplicate in PCR assay)