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. 2008 Jun 24;5(6):e137. doi: 10.1371/journal.pmed.0050137

Figure 4. Inflammatory Changes in A2BAR−/− Mice during Renal Preconditioning.

Figure 4

A2BAR−/− mice and their respective age-, weight-, and gender-matched littermate controls (WT) were subjected to right nephrectomy followed by 45 min of left renal artery ischemia with or without prior in situ IP (consisting of four cycles of 4 min ischemia followed by 4 min reperfusion).

(A–E) Chloracetate esterase staining. Arrows indicate granulocytes (magnification 600×).

(F) Quantification of granulocyte accumulation.

(G) Quantification of neutrophil tissue accumulation by measurement of myeloperoxidase (MPO).

Statistics: (F) Kruskal-Wallis nonparametric analysis (chi-square = 17.86, df = 4, p = 0.001) with follow-up pairwise comparisons by Wilcoxon-Mann-Whitney test was performed with *, p < 0.01 versus WT + IP and **, p < 0.01 versus the A2BAR−/− mice with IP.

(G) One-way ANOVA with Tukey's post-hoc test was performed (*, p < 0.001 versus WT + IP; **, p < 0.001 versus the A2BAR−/− mice with IP). The F-test result is F (4,22) = 46.26, p < 0.001.