A2BAR−/− mice and their respective age-, weight-, and gender-matched littermate controls (WT) were subjected to right nephrectomy followed by 45 min of left renal artery ischemia with or without prior in situ IP (consisting of four cycles of 4 min ischemia followed by 4 min reperfusion).
(A–E) Chloracetate esterase staining. Arrows indicate granulocytes (magnification 600×).
(F) Quantification of granulocyte accumulation.
(G) Quantification of neutrophil tissue accumulation by measurement of myeloperoxidase (MPO).
Statistics: (F) Kruskal-Wallis nonparametric analysis (chi-square = 17.86, df = 4, p = 0.001) with follow-up pairwise comparisons by Wilcoxon-Mann-Whitney test was performed with *, p < 0.01 versus WT + IP and **, p < 0.01 versus the A2BAR−/− mice with IP.
(G) One-way ANOVA with Tukey's post-hoc test was performed (*, p < 0.001 versus WT + IP; **, p < 0.001 versus the A2BAR−/− mice with IP). The F-test result is F (4,22) = 46.26, p < 0.001.