Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Transformation Efficiencies Method
|
Typical Transformation Efficiency (transformants per microgram DNA) |
||
|---|---|---|
| Method |
Auxotrophic Marker Selection |
Zeocin Selection (100 μg/mL) |
| Electroporation | 1 × 105 | 4 × 104 |
| Heat Shock | 1 × 103 | 0–20 |
| Condensed Protocol | 3.5 × 104 | 2 × 103 |
