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. Author manuscript; available in PMC: 2008 Aug 8.
Published in final edited form as: Biochemistry. 2008 May 17;47(23):6138–6147. doi: 10.1021/bi800182t

FIGURE 1.

FIGURE 1

ABCA1 and SPTLC1 interact at physiologic expression levels in macrophages and in the liver. (A) Human THP-1 macrophages differentiated with PMA (100 nM, 72 h) and treated with LXR agonist T0-901317 (10 μM, 24 h) express both ABCA1 and SPTLC1 as determined by immunoblotting (left panels). ABCA1 and SPLTC1 form a protein complex as determined by immunoprecipitation of lysates with an anti-ABCA1 antibody, or an equivalent amount of nonimmune IgG, from differentiated cells treated or not with T0-901317 and immunoblotted for the presence of SPTLC1 in the precipitates (right panel). (B) ABCA1 and SPTLC1 are expressed in mouse liver and interact as determined by immunoblotting (left panels) and immunoprecipitation with an anti-ABCA1 antibody (top right panel) or with an anti-SPTLC1 antibody (bottom right panel).