Figure 2.

In vitro association between the Dorsal RHR and Dorsal RHR domain 1 with Tube and Pelle. (A) Dorsal RHR binding to DNA is inhibited by Tube and Pelle. Gel-shift assays were performed with 0.2 μg of partially purified Dorsal RHR (Dorsal47–403) and a labeled zenB oligonucleotide containing a κB site (lane 1). Each protein was purified and assayed at about 0.1 μg (lanes 2, 4, 6, 8) and 0.5 μg (lanes 3, 5, 7, 9). (B) Dorsal RHR domain 1 binding to DNA is also inhibited by Tube and Pelle but not by Cactus. The amount of all proteins used was the same as in A, except that only one dose of 0.5 μg of D-Lis1 protein was assayed.