Table 2.
LexA fusion | β-gal activity/pJGpelle |
---|---|
LexA-DL-RHR | 80 ± 10 |
LexA-Cactus | 3 ± 2 |
LexA-y3-9 | 2 ± 1 |
LexA-y5-5 | 1 ± 1 |
pJGpelle was cotransformed into yeast expressing LexA fusions and a lacZ reporter gene. For each interaction, β-galactosidase (β-gal) activity was determined for at least three independent clones. Values were normalized to the basal level seen with LexA fusions and the pJG4-5 vector.