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Analysis of binding to left and right operators by EMSA. The 35 bp oligonucleotide duplexes (240 nM) containing the native operator (WT), the left-hand operator only (OL) or the right-hand operator only (OR) were incubated with C.Esp1396I at 0, 1, 2, 3, 4, 5, 6, 7 and 8 : 1 protein:DNA ratios. F = free DNA; D = dimer–DNA complex; T = tetramer–DNA complex. The sequences of the mutated operator duplexes are given in Materials and methods section.
