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. 2008 Jul 21;36(14):4778–4787. doi: 10.1093/nar/gkn448

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Analysis of E25 and R35 mutants. Wild-type C.Esp1396I, and mutant proteins E25A and R35A were analysed by EMSA. Proteins were incubated with a 35 bp dsDNA fragment (240 nM) corresponding to the native operator sequence at protein:DNA ratios of 0, 1, 2, 3, 4, 5, 6, 7 and 8:1. Free DNA (F), dimer–DNA complex (D) and tetramer–DNA (T) complex are indicated. The E25 mutation causes the formation of dimers rather than tetramers. The R35 mutation abolishes all specific nucleoprotein complexes.