Figure 2.

Autoradiogram of in vitro cleavage assays showing the cleavage specificity of the SOFA-HDV Rz directed against the human Pax-5B transcript. SOFA-HDV Rz were tailored against the human Pax-5B mRNA (above sequence) to target nucleotide positions 75, 78, 89, 93 and 97 of the exon 1 region (black arrows). In vitro cleavage assays were performed using 5′ terminal [³²P]-labeled RNA regions of the human Pax-5A and Pax-5B transcript incubated with Pax-5B-specific SOFA-HDV Rz-B75, -B78, -B89, -B93 and -B97 and then migrated on to a 5% polyacrylamide gel and autoradiographed. On the right of the panel, ‘S’ indicates the substrates (∼600 bases), ‘Rz’ indicates the SOFA-HDV Rz and ‘P’ indicates the 5′ terminal cleaved products (∼75 and 78 bases).