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. 2008 Jul 10;36(14):4609–4620. doi: 10.1093/nar/gkn432

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Pax-5B tailored SOFA- HDV Rz cleave their intracellular target substrate in a dose-dependent manner. Quantitative RT–PCR was performed on the 293-B2 cell line (293 HEK cells stably expressing Pax-5B) transiently transfected with increasing amounts of SOFA-HDV Rz-B75 DNA to determine (A) Pax-5B transcripts and (B) SOFA-HDV Rz expression levels. Pax-5B and SOFA-HDV Rz levels were normalized using HPRT mRNA and plotted in relation to the relative percentage of the control 293-B2 cell line. Control samples are also included being the 293-B2 cell line transfected with the SOFA-HDV Rz empty vector (identified psiRNA) and a non-specific SOFA-HDV Rz (identified NS Rz) were also tested. Error bars are indicative of two independent sets of experiments.