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NMR titration experiments focused on the resonances of aromatic side chains in PARN RRM at 25°C. The chemical shift changes, ▵δ, were obtained by calculating the absolute difference of the 1H chemical shifts in the absence and presence of an excess of the cap analog. Resonances of aromatic side chains with chemical shift changes above the mean value (continuous line) are colored cyan, and those with chemical shift changes above the mean value +1 SD (dashed line) are colored green.
