Figure 5.

Pull-down assays of PARN RRM point mutants. The wild-type (WT) and mutant PARN RRM proteins were incubated with m⁷G(5′)ppp-Sepharose 4B beads, and the bound proteins were eluted by the addition of 2× SDS gel loading buffer. These supernatants were collected, and the proteins were fractionated by 15–25% SDS–PAGE and visualized by staining with Coomassie brilliant blue.