Figure 3.
Correlation of reduced in vitro binding affinity of an oligonucleotide containing a variant oriT sequence and reduced transfer efficiency of a plasmid containing the variant sequence. The data for the variants are in black, except two outliers (T141′G and T141′G/G140′A) that are marked in blue. Note that T141′G is included even though its transfer efficiency could not be measured in all assays. The dashed line represents a power law fit to the data excluding the outliers performed using KaleidaGraph. The equation for the fit is y = 0.05186*x⁁(2.7535) (R = 0.6451).