Table 2.

Steady state kinetic constants for the ATP hydrolysis at 80°C

	KM (mM)	Kcat (s−1)	Kcat/KM (mM−1s−1)
TopRI	77.1 ± 19.4	2.55 ± 0.16	3.30 × 10−2
TopRI (no DNA)	16.2 ± 10.2	0.05 ± 0.01	7.00 × 10−3
Nter	775.7 ± 339.3	0.35 ± 0.07	1.89 × 10−4
Nter + Cter	61.9 ± 31.2	0.77 ± 0.09	1.24 × 10−2