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. 2008 Jun 26;36(14):e89. doi: 10.1093/nar/gkn397

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Course of the multiple segmental labeling. (A) The two-step multiple segmental labeling. Lanes 1–8 contain the following samples: (1) 1 µg G9-U28 RNA, (2) 1 µg G1-A8 RNA, (3) 1 µg 28 nt ssDNA splint, (4) 28 nt ssDNA splinted ligation of G1-A8 RNA to G9-U28 RNA (5) purified G1-U28 RNA, (6) 1 µg G29-C61 RNA, (7) G1-U28 RNA to G29-C61 RNA ligation (8) 1 µg of purified G1-C61 ε-DHBV RNA. (B) The one-pot ligation of G1-A8 RNA, G9-U28 RNA and G29-C61 RNA into G1-C61 ε-DHBV RNA. Bands are visualized with Stains-All (Acros).