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. 2008 Jul 16;36(14):4736–4744. doi: 10.1093/nar/gkn424

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Addition of a ternary complexes (green) to a P_i state of 80S ribosomes. (a) Binding of the ternary complex eEF1A·GTP·Val-tRNA to 80S ribosomes in the P_i state carrying an AcPhe-tRNA (red line) at the P site in the presence of MFVK-mRNA. (b) Di-peptide formation in the assay shown in (a). (c) eEF1A·GDPNP·Val-tRNA (green bars) is added to P_i states carrying AcPhe-tRNA (red bars). ν, tRNA bound per ribosome in a binding assay or acylated tRNA per ribosome reacting with puromycin.