Figure 3.

Characterization of recombinants. Lane 1 contains the products generated from a colony PCR reaction with the untreated parental plasmid, subjected to gel electrophoresis on a 1% agarose gel. Lane 2 contains PCR products generated from pSupFG1 plasmid containing only one copy of the supF gene. Lane 3 contains 100 bp DNA ladder. The slower migrating product indicates a gene conversion event, i.e. two copies of the supF gene and the intervening DNA sequence. The faster migrating product runs at the same position as the single copy pSupFG1 plasmid, indicating a recombinant generated by SSA. All recombinant samples shown are from ICL treatment groups.