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HR frequencies induced by TFO-directed ICLs on plasmid p24BstB/TFOLK/BsrB in HeLa cells. (A) Recombination frequencies were measured 48 h after plasmid p24BstB/TFOLK/BsrB alone (duplex), or treated with pTFO1 and UVA irradiation (ICL), were transfected into HeLa cells. Error bars represent SDs. (B) Representative colony PCR analysis of recombinants (as described in Figure 3). Lanes 1–3 contain the same samples as described in Figure 3, lanes 1–3.
