Skip to main content
. 2008 Aug;59(11):3187–3201. doi: 10.1093/jxb/ern175

Fig. 1.

Fig. 1.

Species-specific and developmental expression of NaStEP and NaSoEP. (a) Detection of NaStEP in different genetic backgrounds. Total RNA (10 μg) from mature pistils of SI N. alata (Na) and SC N. plumbaginifolia (Np), N. longiflora (Nl), and N. tabacum (Nt) was blotted and hybridized with 32P-labelled NaStEP. (b) NaStEP expression in developing anthers and pistils of N. alata SC10SC10. Total RNA (10 μg) was loaded in each lane, blotted, and probed with 32P-labelled NaStEP. (c) NaSoEP expression in developing anthers and pistils of N. alata SC10SC10. Total RNA (10 μg) was loaded in each lane, blotted, and probed with 32P-labelled NaSoEP. (d) Densitometric analysis of NaStEP and NaSoEP expression during the development of styles plus stigmas. Numbers 1–5 represents the developmental stages of stigmas plus styles: 1, 0.5–1.0 cm; 2, 1–2 cm; 3, 2–3.5 cm; 4, 3.5–6 cm; and 5, 6 cm. (e) NaStEP and NaSoEP expression in non-sexual organs of N. alata. Total RNA (10 μg) was loaded in each lane, blotted, and probed with a 32P-labelled NaStEP (upper) or NaSoEP (lower). The same amount of stigma RNA was loaded as hybridization control. To ascertain equal RNA loading, blots were stained with methylene blue (lower sections of a–c and e).