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. 2008 Aug;59(11):3187–3201. doi: 10.1093/jxb/ern175

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© 2008 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 1. — Species-specific and developmental expression of NaStEP and NaSoEP. (a) Detection of NaStEP in different genetic backgrounds. Total RNA (10 μg) from mature pistils of SI N. alata (Na) and SC N. plumbaginifolia (Np), N. longiflora (Nl), and N. tabacum (Nt) was blotted and hybridized with ³²P-labelled NaStEP. (b) NaStEP expression in developing anthers and pistils of N. alata S_C10S_C10. Total RNA (10 μg) was loaded in each lane, blotted, and probed with ³²P-labelled NaStEP. (c) NaSoEP expression in developing anthers and pistils of N. alata S_C10S_C10. Total RNA (10 μg) was loaded in each lane, blotted, and probed with ³²P-labelled NaSoEP. (d) Densitometric analysis of NaStEP and NaSoEP expression during the development of styles plus stigmas. Numbers 1–5 represents the developmental stages of stigmas plus styles: 1, 0.5–1.0 cm; 2, 1–2 cm; 3, 2–3.5 cm; 4, 3.5–6 cm; and 5, 6 cm. (e) NaStEP and NaSoEP expression in non-sexual organs of N. alata. Total RNA (10 μg) was loaded in each lane, blotted, and probed with a ³²P-labelled NaStEP (upper) or NaSoEP (lower). The same amount of stigma RNA was loaded as hybridization control. To ascertain equal RNA loading, blots were stained with methylene blue (lower sections of a–c and e).