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. 2008 Aug;59(11):3187–3201. doi: 10.1093/jxb/ern175

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© 2008 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 6. — Immunohistochemical localization of NaStEP in stigmatic tissues of SC N. alata Breakthrough. (a) Stigma and style treated with pre-immune serum. (b) Stigma and style treated with anti-NaStEP antibody (green). (c) Stigma and style treated with anti-HT-B antibody (magenta). (d) and (e) Diagrams of stigma plus style of N. alata and a transverse section of a stigma. The arrow and square show zones where images were taken. (f) Stigma cross-sections showing NaStEP (magenta) in papillary cells (red arrow), secretory cells (yellow arrow), basal cells (blue arrow), and in a portion of the upper transmitting tract. (g) High magnification view of stigmatic secretory cells shows that NaStEP is in small bodies (blue arrows) and in the proximity of the cell wall (green arrows). All figures represent merges of immunostained and phase contrast images of stigmas plus styles. CW, cell wall; V, vacuole; TT, transmitting tissue; UTT, upper transmitting track; C, cortex; PC, papillae cell. Bars, 50 μm for (a), (b), (c) and 20 μm for (f) and 5 μm for (g).