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. 2008 Jul 4;59(11):3051–3068. doi: 10.1093/jxb/ern154

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© 2008 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 2. — Compartments labelled by positively charged nanogold during time-course experiments. (A, B) Labelled MVB fusing with the vacuole (*). The particles were seen in MVBs (see arrows). (C) No nanogold staining was observed in systems of interconnected vesicles forming a single compartment after 15 min of incubation in time-course experiments (arrow: interconnections). (D) Labelling appeared in these compartments after 30 min of incubation with positively charged nanogold and gold particles appeared to detach from the membrane (see arrows). Bar=100 nm.