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. 2008 Jul 4;59(11):3051–3068. doi: 10.1093/jxb/ern154

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© 2008 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 8. — Compartments labelled by positively charged nanogold and quantitation of the labelled profile during the Ikarugamycin experiment: protoplasts were incubated with IKA to inhibit clathrin-dependent endocytosis. (A) Nanogold accumulated on the plasma membrane and in some areas gold labelling formed clusters immediately below the plasma membrane (see arrows). (B, C) Some nanogold particles were observed in interconnected vesicle compartments (B, see arrow) and MVBs (C, see arrow). (D) Quantitation of particle distribution confirmed TEM observations. Since IKA was suspended in DMSO, the effects of IKA on nanogold internalization were compared with DMSO. IKA inhibited probe internalization and many particles were still found in inner vesicles after IKA treatment, suggesting that most of the traffic involving small inner vesicles was due to clathrin-independent processes bypassing tubular/round vesicles. Standard error (SE) is shown for quantitation data. Bar=100 nm.