Fig. 4.

Tissue specificity of MIPS and seed storage proteins in developing seeds of Arabidopsis as determined by immunoblot analysis with MIPS2, 2S albumin, and 12S globulin antibodies. Developing seeds (torpedo to mature stages) were separated into seed coat (Sc) and seed contents which were either left intact as endosperm and embryo (En+Emb), or washed to remove the endosperm (Emb). p2S and 2S are pro- and mature forms of 2S albumin, and p12S and 12S are pro- and mature forms of 12S globulin, respectively. The arrowhead at 60 kDa indicates MIPS.