Figure 1. Paclitaxel and nocodazole but not arsenite disrupt adherent cell tubulin architecture after 1 h.
A. TR9-7 and B. HeLa cells were grown on poly-D-lysine coated coverslips and treated with either nocodazole, paclitaxel or arsenite at the concentrations shown for 1 h before fixation and subsequent staining for cytoskeletal proteins α-tubulin. Cells were analyzed and photographed via fluorescence microscopy. C. Both cell lines were treated with 0 or 5 μM arsenite for 1 h and total cellular lysate proteins were resolved by SDS-PAGE, transferred to nitrocellulose membranes, and probed for HSP70i and β-actin.