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. Author manuscript; available in PMC: 2008 Aug 8.
Published in final edited form as: Biochemistry. 2006 Feb 21;45(7):2380–2386. doi: 10.1021/bi051830y

Figure 1.

Figure 1

Fluorescence emission spectra of [PA63]7*FRET and PA83 control. Fluorescence emission spectrum of [PA63]7*FRET (100 nM; dashed line) prepared by mixing equal parts nPA83 K563C*488 + nPA83 K563C*546 and allowing it to oligomerize on an anion exchange column followed by purification from PA83 and PA20. Fluorescence emission spectrum of PA83 prepared by mixing equal parts PA83 K563C*488 and PA83 K563C*546 (100 nM total PA83; solid line). Excitation using 488 nm line of Ar+ laser. Emission bandpass was 8 nm for both spectra.