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. Author manuscript; available in PMC: 2008 Aug 8.
Published in final edited form as: Biochemistry. 2006 Feb 21;45(7):2380–2386. doi: 10.1021/bi051830y

Figure 5.

Figure 5

Dissociation of [(PA63)2·LFN]. [(PA63)2 LFN]*FRET was rapidly diluted from 5 μM to a final concentration of 10 nM. A decrease in FRET was monitored over time by measuring the fluorescence emission ratio (lower plot; 570 nm / 520 nm; 488 nm excitation). All data were fitted to a single-exponential decay model to recover kd. Residuals were plotted (upper plot). Kinetic transients were acquired at 20°C.