Table 1.

Summary of all DNA sequences used for the ligation reaction, nanoparticle-enhanced SPRI detection and PCR amplification

Array probes (Immobilized on the DNA microarray):

PG = 5′-S-S-(CH2)6-(T)20 GTG CTT TGT TCT GGA TTT CG -3′

PC = 5′-S-S-(CH2)6-(T)20 GTG CTT TGT TCT GGA TTT CC -3′

PA = 5′-S-S-(CH2)6-(T)20 GTG CTT TGT TCT GGA TTT CA -3′

PN = 5′-S-S-(CH2)6-(T)40 -3′

Oligonucleotide Target (Location of the targeted single base pair is underlined):

T = 5′-TGC CCT TGA GGA CCT GCG AAA TCC AGA ACA AAG CAC -3′

Ligation Probe:

L = 5′-PO4 CAG GTC CTC AAG GGC A -3′

Ligation Probe Complement (Attached to gold nanoparticles):

LC = 5′-S-S-(CH2)6-(T)20 TGC CCT TGA GGA CCT G -3′

PCR primers for exon 13 of BRCA1 gene:

Forward: 5′-AAT GGA AAG CTT CTC AAA GTA -3′

Reverse: 5′-ATG TTG GAG CTA GGT CCT TAC -3′