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. 2008 Aug 12;6(8):e194. doi: 10.1371/journal.pbio.0060194

Figure 3. MSI1 and RBR1 Bind to the MET1 Promoter.

Figure 3

(A) Schematic diagram of the MET1 locus representing the fragments (black rectangles) analyzed by PCR after ChIP. White boxes represent the 5′ UTR, and the gray arrow corresponds to the first amino acid of exon 1 of MET1. A putative E2F binding site is represented by a black dot.

(B) ChIP analysis using antibodies specific for MSI1 (MSI1 Ab) and RBR1 (RBR1 Ab) proteins. Nuclear extracts were prepared from wild-type Columbia buds after cross-linking. The first lane represents the input DNA. Control IgG is used as a negative control, while an antibody against histone 3 (H3) is used as a positive control.

(C) Absolute quantification of the ChIP using Q-PCR for the fragment 2. Error bars represent the standard deviation of two independent PCR reactions.