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. 2008 Aug 12;6(8):e194. doi: 10.1371/journal.pbio.0060194

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© 2008 Jullien et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Percentage of ovules/seeds expressing GUS in plants homozygous (HO) for FIS2-GUS in wild-type (WT) and msi1–2/+ backgrounds. The percentage of ovules/seeds is represented before pollination (BP) and 3 d after pollination (3DAP). Error bars represent the standard deviation. The n number is represented on the top of each bar.

(B and C) Photography illustrating FIS2-GUS expression in the central cell of wild-type (B) and msi1–2/+ (C) ovules. Before pollination, it is not possible to distinguish on morphological bases the gametes carrying the wild-type or the msi1–2 allele. Scale bars represent 50 μm.

(D) RT-PCR on RNAs from seeds that inherited msi1–2 maternally selected on the basis of the overexpression of the fluorescent marker KS117 [28] (5 DAP). GAPDH is used as a control.

(E) Percentage of ovules expressing GUS in plants hemizygous (HE) for FIS2-GUS in wild-type and rbr1–1/+ backgrounds. Error bars represent the standard deviation. The n number is represented on the top of each bar.

(F) FIS2-GUS expression in the central cell of rbr1–1/+ ovules before pollination. Before pollination, it is not possible to distinguish on morphological bases the gametes carrying the wild-type or the rbr1–1 allele. Scale bars represent 50 μm.

(G) RT-PCR on RNAs from rbr1–1–selected ovules showing no FIS2 expression in comparison to wild-type ovules. Selection of rbr1–1 ovules was based on the lack of fertilization and seed development at 3 DAP in contrast to the wild-type ovules that were fertilized. GAPDH is used as a control.