Table 1.

Comparative list of single-cell lysis studies.

lysis technique	application	cell type	platform	reference
laser pulse 532 nm	subcellular sampling of fluorescent dye/dye labelled peptides	RBL, PC12	capillary electrophoresis	Li et al. (2001a)
laser pulse 532 nm	characterization of lysis and poration of cells	PtK2	high-speed imaging	Hellman et al. (2007)
laser pulse 532 nm	characterization of cell lysis method	PtK2	high-speed imaging	Rau et al. (2004, 2006)
laser pulse 532 nm	characterization of cell lysis method	BAF-3	high-speed imaging in microfluidic channel	Quinto-Su et al. (2008)
laser pulse 532 nm	PKA, PKC, CamKII and cdc2K enzyme activity	RBL, Swiss 3T3	capillary electrophoresis	Meredith et al. (2000)
laser pulse 532 nm	PKB enzyme activity	HT1080, NIH 3T3	capillary electrophoresis	Li et al. (2004)
laser pulse 532 nm	PKC, CamKII enzyme activity	RBL	capillary electrophoresis	Li et al. (2001b)
laser pulse 532 nm	characterization and optimization of lysis method	RBL	capillary electrophoresis	Sims et al. (1998)
laser pulse 355 nm	β-galactosidase enzyme assay	mast cells	imaging	He et al. (2005)
nanoscale barbs	characterization of lysis efficiency	HL-60, whole blood	microfluidic chip	Di Carlo et al. (2003)
electrical (20 kV cm−1 10 μs pulse)	mRNA quantification	MCF-7, HMT-3522	PCR	Nashimoto et al. (2007)
electrical (300 V cm−1)	characterization of cell lysis method	AML	microfluidic chip electrophoresis	Munce et al. (2004)
electrical (various conditions 10–20 V/10–60 μm for 200–1000 μs)	characterization and optimization of lysis method	RBL	capillary electrophoresis	Han et al. (2003)
electrical (Tesla coil)	LDH enzyme activity	lymphocytes	capillary electrophoresis	Xue & Yeung (1996)
electrical/hypotonic (600 V cm−1 1 s pulse)	GSH quantification	erythrocytes	microfluidic chip electrophoresis	Gao et al. (2004)
electrical/surfactant (450–900 V cm−1 (DC+AC) 3% pluronic P84)	characterization of serial cell lyses	Jurkat	microfluidic chip electrophoresis	McClain et al. (2003)
sonication/detergent (digitonin)	interferon-γ isoform quantification	NK	capillary electrophoresis	Zhang & Jin (2004, 2006)
detergent (0.1% Triton X-100)	optimization of lysis method	MDCK	capillary electrophoresis	Berezovski et al. (2007)
detergent (0.1% Triton X-100)	α-glucosidase I and II, 1,3-N-acetylgalactosaminyltransferase and β-galactosidase enzyme activity	Sf9, HT29, Arabidopsis embryos	capillary electrophoresis	Shoemaker et al. (2005)
detergent/hypotonic (5 mM SDS)	PKA enzyme activity	C2C12	capillary electrophoresis	Zarrine-Afsar & Krylov (2003)
detergent (multiple: anionic, zwitterionic, non-ionic)	protein–DNA complexes	MDCK	capillary electrophoresis	Pang et al. (2006)
chemical/detergent (3 M GTC, 0.2% SDS or 0.1% SDS)	quantification of insoluble actin	MOLT-3	microfluidic chip	Irimia et al. (2004)
detergent (1% DDM)	β2-adrenergic receptor and phycobiliprotein quantification	Sf9 and PCC 7942	microfluidic chip electrophoresis	Huang et al. (2007)
detergent/hypotonic (25 mM SDS)	farnesyltransferase, endoprotease and methyltransferase activity	4T1 and NIH 3T3	capillary electrophoresis	Arkhipov et al. (2005)
detergent/hypotonic (20 mM SDS)	development and optimization of serial adherent cell lyses	RBL	microfluidic chip-fed capillary electrophoresis	Marc et al. (2007)
chemical (20 mM OH− generated at cathode)	characterization and optimization of lysis method	erythrocytes, HeLa, CHO	microfluidic chip	Di Carlo et al. (2005)