Fig. 8.

Effects of BDNF in slices with sprouting were not altered by NMDA receptor antagonism. A, Spontaneous epileptiform activity was recorded in the inner molecular layer in a slice that had mossy fiber sprouting and was exposed to BDNF (200 ng/ml) for 70 min. Note that there was some variability in the number of repetitive events comprising the epileptiform activity. InA1, there are 13 in the example on theleft and 12 in the example on the right. They were recorded 9 and 10 min, respectively, before slices were exposed to 50 μmd-APV. The epileptiform activity shown in A2 was recorded 30 min after APV was added to the buffer containing BDNF. Calibration (shown inB): 5 mV, 1 sec. B, The spontaneous activity shown in A1, on the left, is illustrated with a different time base. Calibration: 5 mV, 400 msec.