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Schematic representation of the GPI transfer reaction. The GPI transamidase catalyzes substitution of the GPI donor for the C-terminal peptide at the ω site in the N-terminally processed proprotein. a, Proprotein; ω, site of cleavage and GPI addition to the proprotein; b, carbohydrate portion of the GPI donor; c, C-terminal signal peptide; d, transamidase; e, terminal ethanolamine group of the GPI.
