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Effect of hGPI8 on C-terminal processing of miniPLAP. Cotranslational processing of miniPLAP mRNA by microsomes of parental K562, untransfected K, and hGPI8/K transfectants in the absence and presence of HDZ. The microsome preparations were made at different times and fewer microsomes (35 vs. 50 OD) were used in the case of hGPI8/K transfectants. The positions of 28-kDa preprominiPLAP, 27-kDa prominiPLAP, 24.7-kDa GPI-anchored miniPLAP, and the 23.0-kDa miniPLAP hydrazide are shown.
