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. Author manuscript; available in PMC: 2009 Jan 23.
Published in final edited form as: Anal Biochem. 2008 Feb 13;376(1):73–82. doi: 10.1016/j.ab.2008.02.004

Fig. 5.

Fig. 5

Phos-tag™ acrylamide gel electrophoresis separation of phosphorylated and unphosphorylated PhoB. (a) Coomassie Blue stained image of PhoB standards with known [PhoB~P]/[PhoBtotal] ratios separated on a 75-μM Phos-tag™ acrylamide gel. (b) Image of the same gel by autoradiography using a Phosphorimager. All lanes were loaded with 3.3 μg PhoBtotal. Lanes 1–5 contained known fractions of 1, 0.75, 0.50, 0.25, and 0 [32P]phosphorylated PhoB ([PhoB~P]/[PhoBtotal]), respectively. Lanes 6 and 7 contained 3.3 μg PhoB D53A either following a 1-h treatment with [32P]PA or untreated, respectively. (c) Plot of autoradiograph signal intensity versus Coomassie staining intensity. Solid line represents the linear regression analysis of the data.