Fig. 4.

Double immunofluorescence staining of the human ED106 specimen with the Cymbus anti-fibroblast antibody (red) and anti-podoplanin antibody (green) in the proximal (A) and mid part (B). Arrow shows overlapping area in (A) and villous structure in (B). An enrichment of Cymbus positive cells in a villous structure of the ED epithelium is shown in (C), representing a double immunofluorescence staining of the ED with the Cymbus anti-fibroblast antibody (red) and anti-podoplanin antibody (green) (C) and transmitted light picture of (C) (D). Arrows show the central region of a villous structure. Bar 50 μm. ED lumen of the endolymphatic duct, CT interstitial connective tissue, B bone, EP epithelium.