Fig. 5.

Down-regulation of GADD45β mRNA and protein. (A) RT-PCR showing a decrease of gadd45β transcripts in the organ of Corti following inhibition of NF-κB. Shown are 24-h treatments with 25 μg/ml of indicated peptides. In the neg. control reactions, RNA isolated from nontreated samples was amplified without the RT cycle preceding PCR. (B) Representative immunoblot (IB) of total protein lysates, prepared from organs of Corti treated as in A, stained with goat anti-GADD45β antibody; lower panel shows colloidal blue staining of loaded proteins; in the neg. control, the membrane was stained with antigoat secondary antibody directly after blocking.