FIG. 6.
Cultured mouse utricles treated with propantheline and labeled for calmodulin and calbindin. Utricles were cultured for 24 h without propantheline (control; A) or with propantheline at 25 (B), 50 (C), 100 (D), 200 (E), and 2,000 μM (F). S indicates striolar hair cells, while E indicates extrastriolar hair cells. Treatment with increasing doses of propantheline resulted in progressively higher degrees of hair cell loss. Scale bar in F = 30 μm and applies to all panels.