Figure 3.

1-D IEF of Callitrichinae MHC class I molecules. Transfections of Callitrichinae MHC class I PCR-generated cDNAs from S. fuscicollis (A), C. jacchus (B), and L. rosalia (C). Biosynthetically labeled MHC class I molecules were precipitated with an antiserum directed against human β₂-microglobulin (αβ₂), an antiserum generated against denatured MHC class I H chains (αHC), or the monoclonal antibody BB7.7 [mAb(+)]. Transfected MHC class I molecules were precipitated from denatured lysates with the antiserum generated against denatured human H chains. Precipitates were treated with neuraminidase in all cases except one [mAb(−)]. These precipitates were then subjected to 1-D IEF.