FIG. 2.

Survival of transplanted ESCs in RC of ouabain-exposed cochleas. A An H&E-stained radial section through the basal turn of the gerbil cochlea shows the normal profiles of SGNs (arrows) within RC. B Only a few neurons (arrowheads) remain in RC in a ouabain-exposed cochlea 1 month after recovery. C A large number of ESCs survive in RC of an “early post-injury” (see text) cochlea 3 weeks after transplantation. ESCs are easily identified by their irregular shape and large, dark blue staining nuclei. D, E Immunostaining for GFP (green) in a section adjacent to the one shown in C identifies the grafted cells as ESCs. Nuclei were counterstained with bis-benzimide (blue). Scale bar = 50 μm.