Phenotype of the TectaA349D/A349D mouse. Toluidin blue-stained 1-μm-thick sections (A–C), anti-Tecta- (D–F), anti-Tectb- (G–I), and anti-otogelin (J–L) stained cryosections from A, D, G, J wild-type mice, B, E, H, K mice (litter U), and C, F, I, LTectaA349D/A349D mice (litter A1). Arrows indicate the tectorial membrane. Arrow in J shows otogelin staining in the sulcal region of the wild-type tectorial membrane. Scale bars = 100 μm.